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Code

Product Name

MD014

Uracil-DNA GlycosylaseUDG

MD029

Heat Sensitive UDG

MD016

25mM dNTP Mix100mM dAtp:dCTP:dGTP:dTTP=1:1:1:1)

MD016M

25mM dN(U)TP Mix (100mMdATP:dCTP:dGTP:dUTP=1:1:1:1)

MD016L

20/40mM dN(U)TP Mix (100mM dATP:dCTP:dGTP:dUTP=1:1:1:2)

MD017

100mM dATP

MD018

100mM dCTP

MD019

100mM dGTP

MD020

100mM dTTP

MD021

100mM dUTP

MD022

SA-HRP/SA-POD

MD050

SUMO Protease


MD014---Uracil-DNA Glycosylase (UDG)

In order to guarantee the accuracy of the PCR resμlts, it is necessary to prevent nonspecific PCR amplification and pollution. UDG enzyme can catalyze the hydrolysis of uracil glycosidic-containing single-stranded DNA or dU-containing double-stranded uracil and the sugar-phosphate backbone of the DNA chain of the N-glycosidic bond and release of free uracil. The most appropriate temperature for UDG is 50℃, 95℃ inactive. The PCR reaction, the most common is the pollutants in PCR products, pollution prevention heat treatment PCR kit replace dTTP to dUTP, so PCR products contain dU of DNA chain. Increase 50℃ heat preservation steps before the PCR reaction, UDG enzyme in the reaction system can be an existing U-DNA base uracil degradation of pollutants, and under the condition of step then modified the DNA chain rupture, eliminate the pollution of DNA amplification, so to ensure that the resμlts of the specificity and accuracy of amplification. UDG enzymes are inactivated at the same time, wi ll not degrade the product of the new expansion U-DNA.


Application:

  1. PCR cross-contamination control

  2. Site-specific mutagenesis

  3. PCR products clone



MD029--- Heat-labile Uracil-DNA Glycosylase

Thermolabile Uracil-DNA Glycosylase removes uracil from DNA by hydrolyzing the N-glycosylic bond between the deoxyribose and the base leaving an AP (apurinic or apyrimidinic) site. This enzyme (1-10 units) is completely inactivated by a 10 minutes incubation at temperatures greater than 50°C in the 1x reaction buffer as measured in the unit characterization assay.

dNTP Mix ,dN(U)TP Mix,dATP ,dCTP ,dGTP ,dTTP ,dUTP

High purity of dNTPs is the key to success PCR reaction since any contamination woμld affect the sensitivity and yield of PCR reaction. Fapon dNTPs is the highest cost performance-price ratio in similar products. We monitor all production processes and analysis of the product physically and functionally to make sure the purity and stability of our products.


MD016---25mM dNTP Mix(100mM Datp:dCTP:dGTP:dTTP=1:1:1:1)


MD016M---25mM dN(U)TP (100mM dATP:dCTP:dGTP:dUTP=1:1:1:1)


MD016L---20/40Mm   


N(U)TP Mix(100mM dATP:dCTP:dGTP:dUTP=1:1:1:2)

Composed of dATP、dCTP、dGTP and dTTP, the concentration of dNTP is 25mM

  1. to be applied as substrate of DNA polymerase.

  2. to be applied directly in normal PCR and RT-PCR directly, which reduce the contamination probability. The usage is 0.4μl in 50μl reaction system with final concentration 200μM.

  3. to be applied directly without any dilution in PCR.


MD017-MD020---100mM dNTP (A, T, C, G)


MD021---100mM dUTP

The concentration of dATP .dCTP .dGTP .dTTP .dUTP is 100mM.

They are suitable for PCR, real-time PCR, RT-PCR, synthesis of cDNA or normal DNA, primer extend reaction, DNA sequencing, DNA label and other normal molecμlar biology reaction.


MD022---SA-HRP/SA-POD

SA-HRP (HRP-labeled Streptavidin) coμld be applied in the detection of biotin-labelled antibody, nucleic acid, protein and other molecμlar. Our SA-HRP is generated by high purity Streptavidin and super purity HRP, which ensure the lowest background and highest sensitivity.

HRP coμld be applied in generating chemiluminescence in Western, EMSA, Southern or Northern when catalyzing ECL reagents such as ECL, Beyo ECL and etc... It is visible as blue when catalyzing TMB in EIA and causes brown precipitation in immunohistochemistry, immunocytochemistry or Western blot


MD050-SUMO Protease

SUMO Protease recognizes the tertiary structure of SUMO rather than an amino acid sequence and therefore it can be used to remove the fusion tags from recombinant proteins. Although the optimal temperature for cleavage is 30°C, it remains active over a wide range of temperature (4-30°C) and pH (pH 7.0-9.0). The SUMO Protease can be removed easily from the cleavage reaction by affinity chromatography using the polyhistidine tag.