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Isothermal Amplification

Code

Product Name

MD030

Bst DNA Polymerase

MD031

Phi29 DNA Polymerase


MD030--- Bst DNA Polymerase

Fapon Bst DNA polymerase large fragment is part of the bacterium Bacillus stearothermophilus DNA polymerase, having 5'→3' DNA polymerase activity, but does not have 5' →3' exonuclease activity. Bst DNA polymerase large fragment with strong strand displacement activity, optimum temperature is 60℃-65℃. By recombinant DNA technology, the Bst DNA polymerase large fragment and label protein are fusion expressed in E.coli. system, label protein resected after purification, then target Bst DNA polymerase large frag mentis collected after second purification.


MD031--- Phi29 DNA Polymerase

Fapon Phi29 DNA Polymerase is a highly persistent polymerase which amplification capability reach up to 70KB, has a strong strand displacement activity for efficient isothermal DNA amplification. Phi29 DNA Polymerase also has a preference for single-stranded DNA 3'→5' exonuclease proofreading function as well as special and continuous strand displacement synthesis features.