Taq

(MD001)

Platinum

(MD002)

Anstart Taq

(MD006)

Taq II DNA Polymerase

(MD0032)

AnPfu

Hot start

(MD036)

Hot start

HItaq

(MD026)

Anstart Taq DNA II Polymerase

(MD007)

Performance

Speed (Max in Internal test)

4KB/1min;

(1KB/5s*)

4KB/1min;

(1KB/5s*)

4KB/1min;

(1KB/5s*)

1KB/30s

1KB/min

1KB/min

Heat activation

95℃

2.5mins

95℃

2.5 mins

95℃

2.5 mins

95℃

2.5 mins

95℃

10 mins

95℃

10 mins

Hot start

√

√

√

Amplicon size

gDNA

3.2KB

3.2KB

Plasmid/lambda

3KB

3KB

3KB

3KB

3KB

cDNA

5KB

5KB

5KB

8KB

5KB

5KB

Application

Low Copy Number

√

√

√

√

√

√

Routine PCR

√

√

√

√

√

√

T/A cloning

√

√

√

√

√

√

Colony PCR

√

√

√

√

√

√

√

Sequencing

√

√

√

√

√

Genotyping

√

√

√

√

Multiplex PCR

√*

√*

√*

√

RT-PCR

√

√

√

√

Complex Template

√*

√*

√

√

√

GC-Rich

√

√

Blunt -end cloning

√

Mutagenesis

√

Additional Features

5'–3' polymerase

activity

√

√

√

√

√

√

3'–5'exo-nuclease activity

√

Isothermal Related

Bst DNA Polymerase（MD030）

Phi29 DNA Polymerase（MD031）

Performance

Optimal extension temperature

60-65℃

30℃

Heat activation

80℃,20 mins

65℃,10 mins

Specification

Isothermal DNA amplification

Isothermal amplification with Hi-Fi

Sequencing

Trace DNA / GC rich sequencing

Strand replacement/leading strand synthesis/PGSPGD*

2.5 X SYBR Anstart One-Step RT-PCR Mix (MD013G)

Anstart One-Step RT-PCR Mix (MD013)

Hot start HITaq One-Step RT-PCR Mix (MD027)

M-MuLV Reverse Transcriptase

（MD011）

Super

M-MuLV Reverse Transcriptase（MD028）

Tth DNA Polymerase（MD012）

Polymerase

Anstart Taq

Hot start Taq

Hot start HITaq

Detection method

SYBR Green 1

probe

probe

Hot start type

antibody

chemical

chemical

Amplicon size

12KB

12KB

2-3KB

Heat activation

95℃

2.5 mins

Optimal extension temperature

50℃

50℃

60-65℃

Format

1 step

(RNA -Ct)

1 step

(RNA -Ct)

1 step

(RNA -Ct)

Enzyme Materials

Polymerase

Phi29 DNA Polymerase (High Concentration)

MD031H

T4 DNA Polymerase

MD033

HiFi Seq DNA Polymerase

MD063

Klenow Fragment

MD037

Klenow (3′→ 5′ exo-)

MD038L

Bst DNA Polymerase

MD030

Poly(A) Polymerase

FPZ-29

Fast T4 DNA Ligase

MD035

Others

T4 Polynucleotide Kinase

MD034

Proteinase K

MD025

RNase H

MD056

Terminal Deoxynucleotidyl Transferase

FPZ-42

Library Construction Preparation Module

DNA Library Construction

End-Repair Module

MD070-E

A-Tailing Module

MD070-A

End-Repair & A-Tailing module

MD073-E

Adapter Ligation Module

MD070-L

WGS Fragmentation Mix

FPZ-04

WGS Ligase

MD065

Library Amplification

HiFi Seq PCR Mix

MD069

Library Preparation Kit

Library Construction

Fast DNA Library Prep Kit for Illumina (Two-step)

MD073

DNA Library Prep Kit for Illumina (Three-step)

MD070

DNA Library Prep Kit for Ion Torrent Platform

FPZ-02

RNA-seq Strand-specific Library Prep Kit

MD079

Single-Cell RNA Sequencing Library Prep Kit

MD075

Library Quantification

Library Quantification Kit for Illumina

MD072

MD014

Uracil-DNA Glycosylase（UDG）

MD029

Heat Sensitive UDG

MD016

25mM dNTP Mix（100mM dAtp:dCTP:dGTP:dTTP=1:1:1:1)

MD016M

25mM dN(U)TP Mix (100mMdATP:dCTP:dGTP:dUTP=1:1:1:1)

MD016L

20/40mM dN(U)TP Mix (100mM dATP:dCTP:dGTP:dUTP=1:1:1:2)

MD017

100mM dATP

MD018

100mM dCTP

MD019

100mM dGTP

MD020

100mM dTTP

MD021

100mM dUTP

MD022

SA-HRP/SA-POD

MD050

SUMO Protease