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Library Preparation Kit

DNA Library Construction

MD073

Fast DNA Library Prep Kit for IlluminaTwo Steps

MD070

DNA Library Prep Kit for Illumina Three Steps

MD079

RNA-seq Strand-specific Library Prep Kit

MD075

Single-Cell RNA sequencing library Prep Kit

FPZ-02

DNA Library Prep Kit for Ion Torrent Platform


MD073-- Fast DNA Library Prep Kit for Illumina (Two Sep)

The Fapon DNA Sample Prep Kit is utilized for the construction of genomic DNA library. All optimized enzymes and buffers are provided with efficient and stable reagents, which can convert 10 ng to 5 ug of fragmented dsDNA into DNA libraries.


Application Scope:

Suitable for the preparation of DNA libraries for Illumina sequencing. Applicable for a variety of sample types and species sources, including a variety of microbial genome, animal and plant genomes, human and mouse genomes. Compatible with the following types of samples: 


  • Genomic DNA

  • REPLI-g amplified DNA

  • Reverse transcription of double-stranded cDNA.

  • PCR Amplicons

  • Cell-Free DNA(cfDNA,ctDNA)

  • DNA extracted from FFPE samples

  • ChIP Seq



Items

20 Reactions

End Repair & A tailing

HiFi Sep DNA Amplification Mix

250 ul

10xPrimers Mix

120 ul

Adapter ligation

Ligation Buffer

750 ul

Ligation Enzyme

650ul


Performance:

  • Shorten preparation time of DNA library to 3 hours. 

  • Less input of DNA templates is required

  • Product quality compatible with Kapa DNA library prep kit after a comparative test on conventional genome library construction.

MD070--DNA Library Prep Kit for Illumina ( Three Steps )

The Fapon DNA Sample Prep Kit is utilized for the construction of genomic DNA library. All optimized enzymes and buffers are provided with efficient and stable reagents, which can convert 10 ng to 5 ug of fragmented dsDNA into DNA libraries.


Application Scope: Suitable for the preparation of DNA libraries for Illumina sequencing. Applicable for a variety of sample types and species sources, including a variety of microbial genome, animal and plant genomes, human and mouse genomes. 


Compatible with the following types of samples: 

  • Genomic DNA

  • REPLI-g amplified DNA

  • Reverse transcription of double-stranded cDNA.

  • PCR Amplicons

  • Cell Free DNA(cfDNA,ctDNA)

  • DNA extracted from FFPE samples

  • ChIP Seq



Items

20 Reactions

End Repair

End Repair Buffer 

250 ul

End Repair Enzyme Mix

100 ul

A Tailing

5×A-Tailing Buffer 

250 ul

A-Tailing Enzyme Mix

120 ul

Adapter ligation

2×Ligation Buffer

600 ul

Ligation Enzyme

70 ul


Fapon three-step DNA library prep kit adopted the classical protocol of library construction. Purification at the end of each step to improve DNA purity, ensuring the efficiency of Library conversion to the maximum degree. Quality of Fapon’s kit equals to Kapa DNA library prep kit after a comparative test on conventional genome library construction.

MD079 -- RNA-seq Strand-specific Library Prep Kit

Fapon RNA Library Prep Kit is applied to transcriptome library preparation on Illumina high-throughput sequencing platform. All optimized enzyme and buffer are stored in a form of reagent. Only 0.1-4ug total RNA is required to construct RNA library.


Application Scope: 0.1-4ug human/animal/plant/microorganisim total RNA with good integrity. rRNA which is often not of interest in total RNA, can be removed using oligo dT beads, or can be depleted using rRNA specific probes.




Items

Fragmentation&

random primers

Fragmentation Buffer

Random Primers

First strand synthesis

First Strand Synthesis Reaction Buffer

First Strand Synthesis Enzyme Mix

Second strand synthesis

Second Strand Synthesis Reaction Buffer with dUTP Mix

Second Strand Synthesis Enzyme Mix

End repair and A-tailing

End Repair & A-Tailing buffer 

End Repair & A-Tailing Enzyme Mix

Adaptor ligation

Ligation Buffer

Ligation Enzyme


MD075--Single-Cell RNA Sequencing Library Prep Kit

Single-Cell RNA sequencing library Prep Kit can extract 10pg-10ng high-quality whole cDNA from single to 10 cells. The amplified products can be applied to Life and Illumina platform for library preparation.



Items

20 Reactions

Cell Lysis

Cell Lysis buffer

15μl

RT-Primer

15μl

First Strand Synthesis

RT Buffer

50μl

RTase

20μl

 

 

Second Strand Synthesis & PCR Amplification

Cut Buffer

5μl

Cutter

15μl

Tag Buffer

15μl

Tagger

7.5μl

PCR Buffer

1.25ml

Tag-Primer

10μl

DNA Polymerase

50μl

PCR-Primer

25μl


FPZ-02---DNA Library Prep Kit for Ion Torrent Platform

Preparation of library DNA is an important process for successfμl amplification on Ion Torrent™ platforms and subsequently generates valuable sequencing data. This kit provides room temperature stable reagents to convert double-stranded fragmented DNA into libraries. The DNA Sample Prep Kit is primarily designed for constructing genomic DNA libraries. All enzymes and buffers required are provided as room temperature stable mixes that can convert 10ng to 1μg of fragmented dsDNA into library DNA.


Library Quantification

MD072

Library Quantification Kit for Illumina


MD072-- Library Quantification Kit for Illumina

Library Quantification Kit for Illumina contains all necessary reagents quantifying stream cell with P5&P7 oligonucleotide sequence.


Application Scope:

Genome source - prokaryotic & eukaryotic, microorganism genome/ animal and plant genome/ mouse genome

Sample types - Genomic DNA,REPLI-g amplified DNARNA transcript double strands cDNA Amplicons



Items

Standard

Standard material 1-6 10-fold dilute

Dilution Buffer

Reaction system

SYBR GREEN PCR mix

Enzyme Mix


Low variation between batches, suitable for automatic liquid handling system and quantification of high-throughpu0t samples.