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Library Construction Preparation Module

DNA Library Construction

MD070-E

End-Repair Module

MD070-A

A-Tailing Module

MD073-E

End-Repair & A-Tailing Module

MD070-L

Adapter Ligation Module

MD065

WGS Ligase

FPZ-04

WGS Fragmentation Mix


MD070-E -- End-Repair Module

Fapon DNA End-repair module is applied to repair cohesive ends of fragmental dsDNA and form 5’ phosphorylated and 3’-OH blunt ends. dsDNA amount from 10ng to 5ug can be handled with this End-repair module.


Application Scope: Fragmental gDNA、cDNA、cfDNA、ctDNA and other PCR products after enzymatic or physical shearing.



Items


End-Repair Module

End Repair buffer 


End Repair Enzyme Mix



MD070-A-- A-Tailing Module

Fapon A-tailing module is applied to the adenine adding of 3’ end of fragmental DNA during DNA library construction. dsDNA amount from 10ng to 5ug is manageable with this end-repair module.


Application Scope: DNA processed by end-repair module and blunt-end dsDNA




Items


A-Tailing Module

 

5×A-Addition Buffer


A-Tailing Enzyme


MD073-E -- End-Repair & A-Tailing Module 

This module is applied to repair cohesive ends of fragmental dsDNA and form 5’ phosphorylated and 3’-OH blunt-end. Furthermore, adding an adenine to 3’ end of fragmental DNA, dsDNA amount from 10ng to 5ug is manageable with this End-repair module.


Application Scope: Fragmental gDNA、cDNA、cfDNA、ctDNA and other PCR products after enzymatic or physical shearing.




Items


End-Repair module

End Repair & A-tailing buffer 


End Repair & A-tailing Enzyme Mix



MD070-L—Adapter Ligation Module

Fapon DNA fast ligation module is applied to connect complementary cohesive end or blunt-end dsDNA with adapters. dsDNA amount from 10ng to 5ug can be handled with this End-repair module.


Application Scope: 

1) dsDNA processed with A-tailing module / T vector

2) dsDNA processed with end-repair & A-tailing module / T vector 

3) complementary cohesive end dsDNA 

4) Blunt-end dsDNA




Items


Fast ligation module

Ligation buffer


Ligation enzyme



MD065--WGS Ligase

WGS Ligase is optimized for ligation following WGS Fragmentation. It catalyzes the formation of a phosphodiester bond between the terminal 5'-phosphate and a 3'-hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single-stranded nicks in duplex RNA, RNA, or DNA/RNA hybrid.


FPZ-04---WGS Fragmentation Mix

This mix provides a single-tube solution for library construction for Illumina platforms. The protocol supports fragmentation, end-repair and dA-tailing in a single reaction step, therefore greatly simplifying the workflow, reducing the total reaction time and hands-on time. 


Library Amplification

MD069

HiFi Seq PCR Mix


MD069—HiFi HiFi Seq PCR Mix

HI-FI PCR is usually adopted for picking out DNA fragments with adaptors in an enrichment library, hence, it is critical to reduce bias for improving the coverage of library. Fapon NGS Library Amplification Module is specially developed for DNA library optimization on Illumina sequencing platform. DNA of high fidelity and less sequence bias can be collected after amplification. P5/P7 Primers Mix in content is mixed forward and reverse primers, which is suitable for DNA fragments with P5 or P7 sequence. Please follow the instructions offered by suppliers if you interested in using alternative primers. 


Application Scope: Illumina high throughput sequencing platform



Items


HiFi Seq PCR Mix

HiFi Seq DNA Amplification Mix


10× Primers Mix



Performance: 

  • Qualified stability and activity

  • Hi-Fi and no obvious base bias

  • High sensitivity, as lowest to 1ng DNA sample